Leuconostoc pseudomesenteroides improve microbiota dysbiosis and liver metabolism imbalance, ameliorate correlation between dihydroceramide and strains Firmicutes and Proteobacteria in high fat diet obese mice
Leuconostoc pseudomesenteroides is widely isolated from fermented foods; however, the function in anti-obesity underlying molecular mechanism has rarely been studied. This study aims to explore the role of alterations in gut microbes and liver metabolites mediated by Leuconostoc pseudomesenteroides (Tu) in obese mice for 8 weeks through UPLC/Q-TOF-MS and 16S rRNA sequencing. Our results showed that Tu administration at the dosage of 1 × 109 CFU/day per mouse effectively attenuated the weight of mice, significantly reduced serum lipids, markedly improved fecal lipids output. Tu also ameliorated the lipid profiles in liver and epididymal fat tissues, restored intestinal disorder caused by high-fat diet. Moreover, glycerophospholipid metabolism in liver were remodeled by increased dihydroceramide levels. Surprisingly, the correlation between dihydroceramide and strains Firmicutes and Proteobacteria was found for the first time. Collectively, these findings highlight that Tu could be a potential dietary supplementation for weight control.