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Comprehensive LC-MS/MS-based phytochemical perspectives and osteogenic effects of Uraria crinita


Osteogenesis plays a vital role in the maintenance of bone health. Imbalances in osteogenesis influence the onset of several bone loss-associated diseases. The intake of Uraria crinita (Fabaceae) through dietary supplements is advised for childhood bone dysplasia. This botanical provides edible tonics and detoxifiers, and is also used as a folk beverage. We evaluated the osteogenic effects of a 50% ethanol extract of root of U. crinita on primary human osteoblasts (HObs) and initiated a novel comprehensive phytochemical strategy using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for quality control of this functional food. Two isoflavones, genistein (5) and 5,7-dihydroxy-3′,5′-dihydroxyisoflavone (6), increased alkaline phosphatase activity (differentiation stage); the flavone glycoside, vitexin (1), and the phenolic acid, salicylic acid (2), enhanced the mineralization (mature stage). The isoflavone, 2′-hydroxygenistein (4), possessed high osteogenic potential among isolated compounds in HObs. It promoted osteogenesis-related stages and upregulated the gene expressions in a dose-dependent manner. The major compounds in the active fraction were quantitatively analyzed via phytochemical fingerprint detection. These LC-MS/MS-based phytochemical perspectives can act as reference standards in developing food supplements from U. crinita.

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Supplementary files

Article information

25 Mar 2020
21 May 2020
First published
22 May 2020

Food Funct., 2020, Accepted Manuscript
Article type

Comprehensive LC-MS/MS-based phytochemical perspectives and osteogenic effects of Uraria crinita

Y. Lin, Y. Mao, Z. Imtiyaz, W. Chiou and M. Lee, Food Funct., 2020, Accepted Manuscript , DOI: 10.1039/D0FO00782J

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