Dietary supplementation with polysaccharides from Ziziphus Jujuba cv. Pozao intervene immune response via regulating peripheral immunity and intestinal barrier function in cyclophosphamide-induced mice
ABSTRACT: Ziziphus Jujuba cv. Pozao has been consumed as a traditional fruit with regional characteristics in China for a long history, while the study of polysaccharides from Ziziphus Jujuba cv. Pozao (JP) has been fewer documented. This study aimed to evaluate the effect of oral administrition of JP on cyclophosphamide-induced ICR mice for 28 days. The result showed that oral administration of JP could improve the lymphocyte proliferation in spleens and significantly decrease the proportion of CD3+, CD4+ and the ratio of CD4+/CD8+ in cyclophosphamide-induced mice. JP treatment also increased the levels of IL-2, IL-4, IL-10, IFN-γ, TNF-α, in serum and intestine. Similarly, JP significantly increased the levels of IgA and SIgA, as well as the expressions of Claudin-1 and Occludin in intestine. Furthermore, JP positively regulated the gut microbiota as indicated by the enriched microbiota diversity. In phylum level, the relative abundance of Firmicutes was siginificantly decreased by JP, while Bacteroidetes was increased by JP treatment. More important, the ratio of Firmicutes/Bacteroidetes was significantly increased. In genus level, the abundances of Bacteroidales-S24-7-group, Lachnospiraceae, Alloprevotella, Alistipes and Bacteroides were increased by JP treatment. These results provide evidence for the effect of JP on cyclophosphamide-induced hypoimmunity and intestinal barrier dysfunction in association with its capacity to regulate peripheral immunity and restore intestinal barrier function in cyclophosphamide-induced mice.