Immune-enhancement of oligosaccharides from Codonopsis pilosula on cyclophosphamide induced immunosuppression in mice
As an important edible traditional Chinese medicine, Codonopsis pilosula has good immunomodulation effects. It has been said that the sweeter the C. Pilosula tastes, the better. This study focused on C. pilosula oligosaccharides (CPO), which is the sweetness component of C. pilosula. CPO was obtained through systematic separation and purification (the yield is 14.3%), and the effect of CPO on the immunological activities of immunocompromised mice induced by cyclophosphamide (CTX) was evaluated. The results showed that CPO could increase immune organs indices, phagocytic index and contents of immunoglobulins, stimulate proliferation of splenic lymphocytes (coordinating with ConA and LPS), enhance earlap swelling of DTH reaction, promote productions of NO and cytokines (IL-2 and IFN-γ) and the expressions of corresponding mRNA. In addition, CPO upregulated the protein expression of phosphorylated p38, phosphorylated ERK1/2 and phosphorylated JNK, which indicated CPO might exert immunomodulatory activities through the MAPKs signaling pathway. These findings indicated that CPO was an important immunomodulatory component in C.pilosula and could be developed as immunomodulator in medicine or functional food areas.