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Effect of regular and decaffeinated roasted coffee (Coffea arabica and Coffea canephora) extracts and bioactive compounds on in vitro probiotic bacteria growth

Abstract

The aim of this study was to investigate the effect of coffee species, roast degree and decaffeination on in vitro probiotic bacteria growth, and to identify major coffee compounds responsible for such effect. Six C. arabica and C. canephora extracts (regular medium and dark roasted and decafeinated medium roasted), and five bioactive compounds (chlorogenic acid, galactomannan, type 2 arabinogalactan, caffeine and trigonelline) were individually incorporated into a modified low-carbon broth medium-(mMRS), at different concentrations (0.5 to 1.5% soluble coffee and 0.05 to 0.8mg/mL standard solutions). Inulin and fructooligossacharides (FOS) were used as prebiotic references. MRS and mMRS were used as rich and poor media controls, respectively. The growth of Lactobacillus rhamnosus GG ATCC 53103-(GG), L. acidophilus LA-5-(LA), Bifidobacterium animalis DN-173010-(BA) and B. animalis subsp. lactis BB12-(BB12), as well as the growth inhibition of non-probiotic Escherichia coli ATCC 25922 were evaluated. Differences in growth between mMRS and treatments (∆ Log CFU/mL) were compared by ANOVA and Tukey test, and considered when p ≤ 0.05. Overall, after 48h incubation, medium roasted arabica coffee extract increased the growth of GG, LA and BA (range ∆ Log CFU/mL = 0.5 to 1.8), while dark roasted arabica coffee extract increased BB12 growth (range ∆ Log CFU/mL = 0.9 to 1.7), in a dose dependent manner. Improved performances of GG, LA and BA were promoted by higher polysacharides and CGA concentrations, with better performance for Lactobacillus sp. The tested coffee bioactive compounds promoted poor growth of BB12. Plain caffeine did not promote Bifidobacterium sp. growth and limited the growth of Lactobacillus sp. Regular C. arabica and C. canephora extracts inhibited the growth of E.coli, while the decaffeinated extracts promoted its growth. The present results show that coffee consumption can selectively improve the growth of probiotic strains, thus exerting a prebiotic effect, and show that coffee roasting and decaffeination affect this property and that different strains utilize different coffee components to grow.

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Article information


Submitted
05 Nov 2019
Accepted
03 Jan 2020
First published
06 Jan 2020

Food Funct., 2020, Accepted Manuscript
Article type
Paper

Effect of regular and decaffeinated roasted coffee (Coffea arabica and Coffea canephora) extracts and bioactive compounds on in vitro probiotic bacteria growth

A. Sales, J. DePaula, C. Mellinger , A. Gomes da Cruz, M. A. Miguel and A. Farah, Food Funct., 2020, Accepted Manuscript , DOI: 10.1039/C9FO02589H

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