α-Lactalbumin and chitosan core−shell nanoparticle: resveratrol loading, protection, and antioxidant activity
Resveratrol (RES)-loaded protein-polysaccharide nanoparticles were fabricated by simple electrostatic interactions with oppositely charged α-Lactalbumin (ALA) and chitosan (CHI) with a mass ratio of 5:1 without NaCl addition at pH 6.5. Z-average diameter and zeta-potential values of RES-ALA-CHI nanoparticle were 211.0 nm, and 13.23 mV, respectively. Both TEM and AFM graphs confirmed RES-ALA-CHI nanoparticle were nanoscale, in spherical shape, and dispersed homogeneously. Encapsulation efficiency (EE) and loading amount (LA) of RES in RES-ALA-CHI nanoparticle were 58.86%, and 196.2 μg/mg in the presence of 400 μg/mL RES, respectively. RES was in amorphous form, testified with XRD results. The interaction between RES and ALA-CHI nanoparticle was mainly driven by hydrophobic interaction and hydrogen bonding. Compared to RES (free), the UV light, and heat stability, in vitro bioaccessibility, and antioxidant activity of RES in RES-ALA-CHI nanoparticle were pronouncedly enhanced. The information provided in this study should be of interest to food industry to fabricate robust nanoscale delivery systems with ALA-CHI nanoparticle for RES and other hydrophobic bioactive compounds.