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Genistein inhibits high-fat-induced obesity through miR-222 by targeting BTG2 and adipor1

Abstract

Obesity and diabetes mellitus have become major health problems worldwide. In recent years, genistein has been found to be capable of inhibiting obesity and alleviating insulin resistance. However, the molecular mechanism of genistein against obesity is not fully understood. In this study, we used 3T3-L1 preadipocyte and obese mice as models to explore the molecular mechanism of genistein against obesity. We found that genistein could inhibit obesity and down-regulate the expression of miR-222 in mouse adipose tissue. In 3T3-L1 preadipocytes, treatment with a miR-222 inhibitor or genistein reduced expression of miR-222 and promoted lipid decomposition, while miR-222 treatment increased miR-222 expression and inhibited lipolysis. Moreover, the dual luciferase reporter system confirmed that BTG2 and adipor1 are the target genes of miR-222. Experiments conducted in vitro and in vivo suggest that genistein may regulate lipid metabolism in adipose tissue of obese mice by regulating the expression of miR-222 and its target genes, BTG2 and adipor1. Our findings provide a new epigenetic mechanism underpinning the ability of genistein to resist obesity. These results may provide a reference point for dietary treatment of obesity and type 2 diabetes mellitus.

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Article information


Submitted
28 Apr 2019
Accepted
10 Feb 2020
First published
13 Feb 2020

Food Funct., 2020, Accepted Manuscript
Article type
Paper

Genistein inhibits high-fat-induced obesity through miR-222 by targeting BTG2 and adipor1

M. Gan, L. Shen, S. Wang, Z. Guo, T. Zheng, Y. Fan, Y. Tan, L. Liu, L. Chen, A. Jiang, X. Li, S. Zhang and L. zhu, Food Funct., 2020, Accepted Manuscript , DOI: 10.1039/C9FO00861F

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