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Membrane Electrostatics Sensed by Tryptophan Anchors in Hydrophobic Model Peptides Depends on Non-Aromatic Interfacial Amino Acids: Implications in Hydrophobic Mismatch

Abstract

WALPs are synthetic α-helical membrane-spanning peptides that constitute a well-studied system for exploring hydrophobic mismatch. These peptides represent a simplified consensus motif for transmembrane domains of intrinsic membrane proteins due to their hydrophobic core of alternating leucine and alanine flanked by membrane-anchoring aromatic tryptophan residues. Although the modulation of mismatch responses in WALPs by tryptophan anchors has been reported earlier, there have been limited attempts to utilize the intrinsic tryptophan fluorescence of this class of peptides as mismatch sensors. We have previously shown, utilizing the red edge excitation shift (REES) approach, that interfacial WALP tryptophan residues in fluid phase bilayers experience a dynamically constrained membrane microenvironment. Interestingly, emerging reports suggest the involvement of non-aromatic interfacially localized residues in modulating local structure and dynamics in WALP analogs. In this backdrop, we have explored the effect of interfacial amino acids, such as lysine (in KWALPs) and glycine (in GWALPs), on the tryptophan microenvironment of WALP analogs in zwitterionic and negatively charged membranes. We show that interfacial tryptophans in KWALP and GWALP experience a more restricted microenvironment, as reflected in the substantial increase in magnitude of REES and apparent rotational correlation time, relative to those in WALP in zwitterionic membranes. Interestingly, in contrast to WALP, tryptophan anchors in KWALP and GWALP appear insensitive to the presence of negatively charged lipids in the membrane. These results bring out a subtle interplay between non-aromatic flanking residues in transmembrane helices and negatively charged lipids at the membrane interface, which could modulate the membrane microenvironment experienced by interfacially localized tryptophan residues. Since interfacial tryptophans are known to influence mismatch responses in WALPs, our results point out the possibility of utilizing fluorescence signatures of tryptophans in membrane proteins or models peptides such as WALP as markers for assessing protein responses to hydrophobic mismatch. More importantly, these results constitute one of the first reports on the influence of lipid headgroup charge in fine-tuning hydrophobic mismatch in membrane bilayers, thereby enriching the existing framework of hydrophobic mismatch.

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Supplementary files

Article information


Submitted
18 May 2020
Accepted
11 Sep 2020
First published
21 Sep 2020

Faraday Discuss., 2020, Accepted Manuscript
Article type
Paper

Membrane Electrostatics Sensed by Tryptophan Anchors in Hydrophobic Model Peptides Depends on Non-Aromatic Interfacial Amino Acids: Implications in Hydrophobic Mismatch

S. Pal, R. E. Koeppe II and A. Chattopadhyay, Faraday Discuss., 2020, Accepted Manuscript , DOI: 10.1039/D0FD00065E

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