Effect of C/N on partial nitrification in MBBR at low temperature
Two main problems in partial nitrification at low temperature are hard to start and easy to destroy, and this issue significantly limits the broad application of advance partial nitrification technology. To promote rapid culturing and stabilization of partial nitrification biofilm, two experiments were carried out to investigate the influence of C/N on partial nitrification through ratio control (R, dissolved oxygen(DO)/Effluent total ammonia nitrogen (TANeff)) in the lab-scale moving bed biofilm reactor (MBBR) at 12℃. One experimental looked at the effect of C/N on the stable operation of partial nitrification at 12℃, which inoculated mature biofilm with good partial nitrification performance; the other looked at the effect of C/N on the biofilm cultivation and start-up ofpartial nitrificationat 12℃, which inoculated activated sludge and Pall rings. As for the startup experiments, only the C/N ratio was investigated, as DO/TANeff ratio was kept constant. The effect of C/N on the mature biofilm of partial nitrification showed that the increase of C/N could increase the resistance of partial nitrification to high DO. Nitrite accumulation ratio (NAR, (NO2--N/(NO2--N+NO3--N)×100%)) increased from 40.41% to 98.78% when R was 0.25 and C/N increased from 0 to 1. While C/N increased to 2 and R value slightly increased to 0.3, partial nitrification failed. The penetration of DO in the biofilm indicated that the increase of C/N enhance the mass transfer resistance of DO, but it was similar when C/N was 1 and 2. Adding an appropriate amount of C/N can reduce the negative effect of partial nitrification damage caused by DO/TAN enlargement. The start-up experiment showed that C/N=1 and C/N=2 were essential for culturing biofilm and achieving partial nitrification at 12℃, which can successfully achieve (NAR was 85% and 96%) on day 56th and 52nd. Neither C/N = 0 nor C/N = 3 could effectively achieve the partial nitrification. The increase of C/N will reduce the proportion of nitrifying bacteria in the biofilm and which is harmful to the partial nitrification. It will also lead to the decrease of protein/polysaccharide (PN/PS) and affect the stability of the biofilm.