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A new monooxygenase from Herbaspirillum huttiense catalyzed highly enantioselective epoxidation of allylbenzenes and allylic alcohols

Abstract

Asymmetric epoxidation is a green route to enantiopure epoxides, but often suffers low enantioselectivity toward unconjugated terminal alkenes. Mining of the NCBI non-redundant protein sequences with a reconstructed ancestral sequence based on six styrene monooxygenases identified a monooxygenase (HhMo) from Herbaspirillum huttiense with 29.6-32.3% sequence identity to styrene monooxygenases, which was previously annotated as an alanine phosphoribitol ligase. HhMo catalyzed the epoxidation of allylbenzenes with moderate to excellent enantioselectivity yielding the corresponding epoxides in up to 99% ee. The HhMo-catalyzed epoxidation could also achieve the kinetic resolution of racemic secondary allylic alcohols, yielding the corresponding epoxides with up to 50% yields, as well as excellent enantio- and diastereo-selectivity (up to >99% ee and >99% de) within 20-60 min, making a greener strategy for the production of valuable enantiopure glycidol derivatives in fine chemicals and pharmaceuticals.

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Supplementary files

Article information


Submitted
15 Jan 2020
Accepted
13 Feb 2020
First published
14 Feb 2020

Catal. Sci. Technol., 2020, Accepted Manuscript
Article type
Paper

A new monooxygenase from Herbaspirillum huttiense catalyzed highly enantioselective epoxidation of allylbenzenes and allylic alcohols

H. Lin, Y. Tang, S. Dong, R. Lang and H. Chen, Catal. Sci. Technol., 2020, Accepted Manuscript , DOI: 10.1039/D0CY00081G

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