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Issue 12, 2020

High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

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Abstract

In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRNA display selections. In this Perspective, we introduce aspects of mRNA display and HTS that may be of interest to physical chemists. We highlight the potential of HTS to analyze in vitro selections of peptides and review recent advances in the application of HTS analysis to mRNA display experiments. We discuss some possible issues involved with HTS analysis and summarize some strategies to alleviate them. Finally, the potential for future impact of advancing HTS analysis on mRNA display experiments is discussed.

Graphical abstract: High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

Article information


Submitted
30 Oct 2019
Accepted
13 Jan 2020
First published
17 Jan 2020

This article is Open Access

Phys. Chem. Chem. Phys., 2020,22, 6492-6506
Article type
Perspective

High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

C. Blanco, S. Verbanic, B. Seelig and I. A. Chen, Phys. Chem. Chem. Phys., 2020, 22, 6492 DOI: 10.1039/C9CP05912A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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