Synthesis of Biocompatible Nanoporous Zeolitic imidazolate Framework-8 in the Presence of Gum Arabic Inspired by the Biomineralization Process
Here by inspiration from the natural biomineralization process, the one-pot synthesis of Zeolitic Imidazolate Framework-8 (ZIF-8, containing Zn2+ as metal center and 2-methyl imidazole as linker) was done in the presence of polysaccharide (Gum Arabic, GA), which enhanced its biocompatibility for application in the drug delivery systems. From the best of our knowledge it is the first report about the synthesis of ZIF-8 in the presence of Gum Arabic. The effect of some parameters such as concentration of Gum Arabic, linker/metal molar ratio and water amount was investigated on the size, morphology and crystallinity of the synthesized structures. The products were characterized with field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), Brunauer-Emmett-Teller (BET) surface area ,and thermogravimetric analysis (TGA) and UV-Vis spectroscopy techniques. In addition, the standard 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed on the human normal Fibroblast cell line, to compared the cytotoxicity of the ZIF-8 and ZIF-8-GA structures. The results showed the enhanced biocompatibility of ZIF-8-GA compared to ZIF-8 due to the presence of GA in the ZIF-8 framework.