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Issue 98, 2020
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Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

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Abstract

The bottom-up construction of bio-inspired systems capable of self-maintenance and reproduction is a central goal in systems chemistry and synthetic biology. A particular challenge in such systems is the continuous regeneration of key proteins required for macromolecular synthesis. Here, we probe self-maintenance of a reconstituted in vitro translation system challenged by serial transfer of selected key proteins. We find that the system can simultaneously regenerate multiple essential polypeptides, which then contribute to the maintenance of protein expression after serial transfer. The presented strategy offers a robust methodology for probing and optimizing continuous self-regeneration of proteins in cell-free environments.

Graphical abstract: Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

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Supplementary files

Article information


Submitted
28 Sep 2020
Accepted
18 Nov 2020
First published
19 Nov 2020

This article is Open Access

Chem. Commun., 2020,56, 15426-15429
Article type
Communication

Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

K. Libicher and H. Mutschler, Chem. Commun., 2020, 56, 15426
DOI: 10.1039/D0CC06515C

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    [Original citation] - Published by The Royal Society of Chemistry.

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