An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines

Yong He; Junli Yu; Xiangzi Hu; Shumei Huang; Lili Cai; Liu Yang; Huatang Zhang; Yin Jiang; Yongguang Jia; Hongyan Sun

doi:10.1039/D0CC06129H

An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines†

Yong He,‡^a Junli Yu,‡^b Xiangzi Hu,‡^a Shumei Huang,^a Lili Cai,^a Liu Yang,^cd Huatang Zhang,

*^a Yin Jiang,*^a Yongguang Jia

^e and Hongyan Sun

*^cd

Author affiliations

* Corresponding authors

^a School of Chemical Engineering and Light Industry and School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China
E-mail: htzhang@gdut.edu.cn, yjiang@gdut.edu.cn

^b Department of Ultrasonography, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

^c Department of Chemistry and Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China
E-mail: hongysun@cityu.edu.hk

^d Chengdu Research Institute, City University of Hong Kong, Chengdu, China

^e National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China

Abstract

Herein, a new fluorescent probe, AE-Phos, is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity, a large Stokes shift, and a fast response towards ALP. Furthermore, AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively.

Chemical Communications

An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines†

Abstract

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An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines

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