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A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

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Abstract

A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.

Graphical abstract: A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

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Article information


Submitted
07 Sep 2020
Accepted
28 Sep 2020
First published
28 Sep 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

W. Tian, X. Liu, G. Wang and C. Liu, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC06034H

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