A dumbbell probe-based dual signal amplification strategy for sensitive detection of multiple DNA methyltransferases

Abstract

We demonstrate for the first time the integration of a dumbbell probe with dual signal amplification for the simultaneous detection of multiple DNA methyltransferases (MTases). This method is very sensitive with a detection limit of 2.15 × 10⁻⁵ U mL⁻¹ for DNA adenine methyltransferase (Dam) and 3.23 × 10⁻⁶ U mL⁻¹ for CpG Methyltransferase (M.SssI), and it can be used to screen the enzyme inhibitors and simultaneously measure Dam and M.SssI in complex biological samples.