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Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

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Abstract

DAPI is used extensively to identify and quantify DNA in cellular systems assuming its exclusive staining of nucleic acids. However, the present results show that DAPI has much higher affinity towards protein aggregates than DNA. Thus, the use of DAPI for the nucleic acid assay of cellular systems with protein aggregates may not be very reliable.

Graphical abstract: Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

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Article information


Submitted
12 Jun 2020
Accepted
13 Oct 2020
First published
13 Oct 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

A. K. Mora, S. Khan, B. S. Patro and S. Nath, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC04108D

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