Issue 89, 2020
From the journal:

Chemical Communications

Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?†

Aruna K. Mora, ORCID logo *a   Sufiyan Khan, ORCID logo b   Birija S. Patro ORCID logo cd  and  Sukhendu Nath ORCID logo *ac  

* Corresponding authors

a Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
E-mail: arunm@barc.gov.in, snath@barc.gov.in

b Ramnarain Ruia Autonomous College, Mumbai 400 019, India

c Homi Bhabha National Institute, Anushaktinagar, Mumbai 400 094, India

d Bio-Organic Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Abstract

DAPI is used extensively to identify and quantify DNA in cellular systems assuming its exclusive staining of nucleic acids. However, the present results show that DAPI has much higher affinity towards protein aggregates than DNA. Thus, the use of DAPI for the nucleic acid assay of cellular systems with protein aggregates may not be very reliable.

Graphical abstract: Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

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Article information

DOI
https://doi.org/10.1039/D0CC04108D
Article type
Communication
Submitted
12 Jun 2020
Accepted
13 Oct 2020
First published
13 Oct 2020

Chem. Commun., 2020,56, 13844-13847

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Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?

A. K. Mora, S. Khan, B. S. Patro and S. Nath, Chem. Commun., 2020, 56, 13844 DOI: 10.1039/D0CC04108D

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