Jump to main content
Jump to site search

Issue 68, 2020
Previous Article Next Article

Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase

Author affiliations

Abstract

By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase. This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases.

Graphical abstract: Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase

Back to tab navigation

Supplementary files

Article information


Submitted
22 May 2020
Accepted
21 Jul 2020
First published
21 Jul 2020

This article is Open Access

Chem. Commun., 2020,56, 9850-9853
Article type
Communication

Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase

A. Djeghader, M. Rossotti, S. Abdulkarim, F. Biaso, G. Gerbaud, W. Nitschke, B. Schoepp-Cothenet, T. Soulimane and S. Grimaldi, Chem. Commun., 2020, 56, 9850
DOI: 10.1039/D0CC03634J

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements