Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Development of a bidirectional isothermal amplification strategy for the sensitive detection of transcription factors in cancer cells

Author affiliations

Abstract

We developed a new strategy to sensitively detect transcription factors (TFs) based on the integration of a bidirectional isothermal exponential amplification reaction (EXPAR) with endonuclease IV (endo IV)-assisted cycle digestion of signal probes. This assay exhibits ultrahigh sensitivity with a detection limit of 1.29 × 10−14 M, and it can measure endogenous NF-κB p50 in HeLa cell extracts. Moreover, this strategy can be applied to screen TF inhibitors and detect other TFs by simply changing the TF-binding sequence.

Graphical abstract: Development of a bidirectional isothermal amplification strategy for the sensitive detection of transcription factors in cancer cells

Back to tab navigation

Supplementary files

Article information


Submitted
30 Apr 2020
Accepted
30 Jun 2020
First published
30 Jun 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Development of a bidirectional isothermal amplification strategy for the sensitive detection of transcription factors in cancer cells

Y. Zhang, Q. Li, D. Xiang, K. Zhou, Q. Xu and C. Zhang, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC03134H

Social activity

Search articles by author

Spotlight

Advertisements