Issue 63, 2020
From the journal:

Chemical Communications

Simple and label-free strategy for terminal transferase assay using a personal glucose meter†

Hyo Yong Kim,a   Jayeon Song,a   Ki Soo Park ORCID logo *b  and  Hyun Gyu Park ORCID logo *a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
E-mail: hgpark@kaist.ac.kr

b Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea
E-mail: kskonkuk@gmail.com

Abstract

We herein developed a simple personal glucose meter (PGM)-based method for terminal transferase (TdT) activity assay by utilizing the glucose oxidase (GOx)-mimicking activity of cerium oxide nanoparticles (CeO2 NPs). Using this strategy, the TdT activity was reliably determined down to 0.7 U mL−1 with high selectivity against other non-specific enzymes.

Graphical abstract: Simple and label-free strategy for terminal transferase assay using a personal glucose meter

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Article information

DOI
https://doi.org/10.1039/D0CC02869J
Article type
Communication
Submitted
21 Apr 2020
Accepted
24 Jun 2020
First published
25 Jun 2020

Chem. Commun., 2020,56, 8912-8915

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Simple and label-free strategy for terminal transferase assay using a personal glucose meter

H. Y. Kim, J. Song, K. S. Park and H. G. Park, Chem. Commun., 2020, 56, 8912 DOI: 10.1039/D0CC02869J

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