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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

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Abstract

By identification of a super-stable protein–DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.

Graphical abstract: High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

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Article information


Submitted
20 Apr 2020
Accepted
01 Jun 2020
First published
01 Jun 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

F. Yu, Z. Yuan, D. Zhang, Y. Liu, Q. Zhao and H. Wang, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC02844D

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