Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

Author affiliations

Abstract

The dynamics of GPCRs (G protein-coupled receptors) coupling for cognate G proteins play a critical role in signal transduction. Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differences of Gαi1 (inhibitory G proteins) and Gαs (stimulatory G proteins) upon β2AR (β2-adrenergic receptor) coupling. It offers a new generally applicable method to probe protein dynamic interactions or conformational changes.

Graphical abstract: A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

Back to tab navigation

Supplementary files

Article information


Submitted
14 Apr 2020
Accepted
13 May 2020
First published
13 May 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

P. Shi, Y. Zhang, P. Lv, W. Fang, S. Ling, X. Guo, D. Li, S. Liu, D. Sun, L. Zhang, D. Liu, J. Zheng and C. Tian, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC02691C

Social activity

Search articles by author

Spotlight

Advertisements