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A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

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Abstract

A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.

Graphical abstract: A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

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Supplementary files

Article information


Submitted
24 Mar 2020
Accepted
17 Jun 2020
First published
18 Jun 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

S. Brands, H. U. C. Brass, A. S. Klein, J. Pietruszka, A. J. Ruff and U. Schwaneberg, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC02181D

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