Issue 46, 2020
From the journal:

Chemical Communications

Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation†

Kosuke Nakamoto, ORCID logo a   Naoko Abe, ORCID logo a   Genichiro Tsuji,ab   Yasuaki Kimura, ORCID logo a   Fumiaki Tomoike, ORCID logo ac   Yoshihiro Shimizu ORCID logo d  and  Hiroshi Abe ORCID logo *ae  

* Corresponding authors

a Graduate School of Science, Nagoya University, Nagoya, Japan
E-mail: h-abe@chem.nagoya-u.ac.jp

b National Institute of Health Sciences, Kanagawa, Japan

c Faculty of Science, Gakushuin University, Tokyo, Japan

d RIKEN Center for Biosystems Dynamics research, Osaka, Japan

e JST CREST, Science and Technology Agency, Tokyo, Japan

Abstract

Circular RNA without a stop codon enables rolling circle translation. To produce circular RNAs, we carried out one-pot chemical synthesis of circular RNA from RNA fragments with the use of an EDC/HOBt-based chemical ligation reaction. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.

Graphical abstract: Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation

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Article information

DOI
https://doi.org/10.1039/D0CC02140G
Article type
Communication
Submitted
23 Mar 2020
Accepted
17 Apr 2020
First published
21 Apr 2020

Chem. Commun., 2020,56, 6217-6220

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Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation

K. Nakamoto, N. Abe, G. Tsuji, Y. Kimura, F. Tomoike, Y. Shimizu and H. Abe, Chem. Commun., 2020, 56, 6217 DOI: 10.1039/D0CC02140G

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