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A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

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Abstract

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30–150, 30–140, and 19–120 fmol per cell, respectively.

Graphical abstract: A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

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Supplementary files

Article information


Submitted
20 Mar 2020
Accepted
01 May 2020
First published
01 May 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

S. Chen, J. Zhao, X. Yang, S. Zhao and Y. Liu, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC02072A

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