Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



An NMR based phosphodiesterase assay

Author affiliations

Abstract

We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.

Graphical abstract: An NMR based phosphodiesterase assay

Back to tab navigation

Supplementary files

Article information


Submitted
03 Mar 2020
Accepted
01 Jun 2020
First published
17 Jun 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

An NMR based phosphodiesterase assay

M. Akimoto, T. Yu, K. Moleschi, K. Van, G. S. Anand and G. Melacini, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC01673J

Social activity

Search articles by author

Spotlight

Advertisements