Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification†
Fujian
Xu, 
‡a
Sichun
Zhang
*a
and
‡a
Sichun
Zhang
*a
and
Abstract
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.
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