Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 54, 2020
Previous Article Next Article

Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

Author affiliations

Abstract

The CRISPR–Cas9 system enables facile and efficient genome engineering in living cells and organisms. We report a Cas9–RNA conjugation strategy to afford minimal crRNA containing only the guide sequence for the target gene, which may simplify and reduce the cost for large-scale and high-throughput crRNA synthesis and lead to new insights into the design of CRISPR family complexes for diverse purposes.

Graphical abstract: Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

Back to tab navigation

Supplementary files

Article information


Submitted
24 Feb 2020
Accepted
26 May 2020
First published
02 Jun 2020

Chem. Commun., 2020,56, 7515-7518
Article type
Communication

Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

X. Ling, X. Gao, L. Chang, H. Chen, X. Shi and T. Liu, Chem. Commun., 2020, 56, 7515
DOI: 10.1039/D0CC01432J

Social activity

Search articles by author

Spotlight

Advertisements