Jump to main content
Jump to site search


A sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with orthogonal color readouts enabled by CRISPR Cas12a

Abstract

Herein, we introduce a sequence-specific plasmonic LAMP assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5’TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.

Back to tab navigation

Supplementary files

Article information


Submitted
15 Jan 2020
Accepted
14 Feb 2020
First published
14 Feb 2020

Chem. Commun., 2020, Accepted Manuscript
Article type
Communication

A sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with orthogonal color readouts enabled by CRISPR Cas12a

R. Zhou, Y. Li, T. Dong, Y. Tang and F. Li, Chem. Commun., 2020, Accepted Manuscript , DOI: 10.1039/D0CC00397B

Social activity

Search articles by author

Spotlight

Advertisements