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Issue 24, 2020
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A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

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Abstract

Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5′-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.

Graphical abstract: A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

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Supplementary files

Article information


Submitted
15 Jan 2020
Accepted
14 Feb 2020
First published
14 Feb 2020

Chem. Commun., 2020,56, 3536-3538
Article type
Communication

A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

R. Zhou, Y. Li, T. Dong, Y. Tang and F. Li, Chem. Commun., 2020, 56, 3536
DOI: 10.1039/D0CC00397B

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