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Improving resolving ability of expansion microscopy by varying crosslinker concentration

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Abstract

Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N,N′-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm with a common confocal microscope.

Graphical abstract: Improving resolving ability of expansion microscopy by varying crosslinker concentration

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Article information


Submitted
03 Jan 2020
Accepted
05 Mar 2020
First published
06 Mar 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Improving resolving ability of expansion microscopy by varying crosslinker concentration

Y. Hu, X. Chu, T. Chen, Q. Pan, C. Liu, J. Yi and X. Chu, Chem. Commun., 2020, Advance Article , DOI: 10.1039/D0CC00052C

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