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Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

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Abstract

We develop a simple fluorescence method for the sensitive detection of cathepsin B activity based on the integration of a peptide–DNA conjugate with multiple cyclic signal amplification. This method can detect cathepsin B activity with an extremely low detection limit of 8.1 × 10−12 g mL−1 and a large dynamic range of 4 orders of magnitude from 1 × 10−11 to 1 × 10−7 g mL−1, and it can even measure cathepsin B activity at the single-cell level. This method can be further used for the screening of cathepsin B inhibitors.

Graphical abstract: Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

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Supplementary files

Article information


Submitted
14 Dec 2019
Accepted
07 Jan 2020
First published
14 Jan 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

Z. Wang, C. Zhang and C. Zhang, Chem. Commun., 2020, Advance Article , DOI: 10.1039/C9CC09714G

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