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Integration of peptide-DNA conjugate with multiple cyclic signal amplification for ultrasensitive detection of cathepsin B activity

Abstract

We develop a simple fluorescent method for sensitive detection of cathepsin B activity based on the integration of peptide-DNA conjugate with multiple cyclic signal amplification. This method can detect cathepsin B activity with an extremely low detection limit of 8.1 × 10-12 g/mL and a large dynamic range of 4 orders of magnitude from 1 × 10-11 to 1 × 10-7 g/mL, and it can even measure cathepsin B activity at the single-cell level. This method can be further used for the screening of cathepsin B inhibitors.

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Supplementary files

Article information


Submitted
14 Dec 2019
Accepted
07 Jan 2020
First published
14 Jan 2020

Chem. Commun., 2020, Accepted Manuscript
Article type
Communication

Integration of peptide-DNA conjugate with multiple cyclic signal amplification for ultrasensitive detection of cathepsin B activity

Z. Wang, C. Zhang and C. Zhang, Chem. Commun., 2020, Accepted Manuscript , DOI: 10.1039/C9CC09714G

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