A highly sensitive signal-on biosensor for microRNA 142-3p based on the quenching of Ru(bpy)32+–TPA electrochemiluminescence by carbon dots and duplex specific nuclease-assisted target recycling amplification

Jiao Yang; Qian Xia; Longhua Guo; Fang Luo; Yongqiang Dong; Bin Qiu; Zhenyu Lin

doi:10.1039/C9CC09706F

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A highly sensitive signal-on biosensor for microRNA 142-3p based on the quenching of Ru(bpy)₃²⁺–TPA electrochemiluminescence by carbon dots and duplex specific nuclease-assisted target recycling amplification†

Jiao Yang,^a Qian Xia,^a Longhua Guo,*^b Fang Luo,

^a Yongqiang Dong,

*^a Bin Qiu

^a and Zhenyu Lin

*^a

Author affiliations

* Corresponding authors

^a Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China
E-mail: dongyq@fzu.edu.cn, zylin@fzu.edu.cn
Fax: +86-591-22866135
Tel: +86-591-22866135

^b College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, China
E-mail: guolh@zjxu.edu.cn

Abstract

Carbon dots (CDs) have been used to quench the electrochemiluminescence (ECL) of a Ru(bpy)₃²⁺–tripropylamine (TPA) system for the first time. In addition, this phenomenon has been coupled with duplex specific nuclease-assisted target recycling amplification to develop a highly sensitive ECL biosensor for microRNA.

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Article information

DOI: https://doi.org/10.1039/C9CC09706F
Article type: Communication
Submitted: 14 Dec 2019
Accepted: 29 Apr 2020
First published: 29 Apr 2020

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Chem. Commun., 2020,56, 6692-6695

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A highly sensitive signal-on biosensor for microRNA 142-3p based on the quenching of Ru(bpy)₃²⁺–TPA electrochemiluminescence by carbon dots and duplex specific nuclease-assisted target recycling amplification

J. Yang, Q. Xia, L. Guo, F. Luo, Y. Dong, B. Qiu and Z. Lin, Chem. Commun., 2020, 56, 6692 DOI: 10.1039/C9CC09706F

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Chemical Communications