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Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule–enzyme hybrid

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Abstract

In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed “Supra-blot” capitalizing on high-affinity host–guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule–enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells.

Graphical abstract: Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule–enzyme hybrid

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Supplementary files

Article information


Submitted
13 Dec 2019
Accepted
06 Jan 2020
First published
06 Jan 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule–enzyme hybrid

G. Sung, S. Lee, M. Kang, K. L. Kim, J. An, J. Sim, S. Kim, S. Kim, J. Ko, H. Rhee, K. M. Park and K. Kim, Chem. Commun., 2020, Advance Article , DOI: 10.1039/C9CC09699J

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