Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment

Thilanga N. Pahattuge; J. Matt Jackson; Rane Digamber; Harshani Wijerathne; Virginia Brown; Malgorzata A. Witek; Chamani Perera; Richard S. Givens; Blake R. Peterson; Steven A. Soper

doi:10.1039/C9CC09598E

Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment†

Thilanga N. Pahattuge,‡^a J. Matt Jackson,‡^a Rane Digamber,^b Harshani Wijerathne,

^a Virginia Brown,^c Malgorzata A. Witek,^a Chamani Perera,^b Richard S. Givens,

^a Blake R. Peterson

^b and Steven A. Soper

*^acde

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* Corresponding authors

^a Center of BioModular Multi-Scale Systems, Department of Chemistry, University of Kansas, 1567 Irving Hill Rd., Lawrence, KS 66045, USA
E-mail: ssoper@ku.edu

^b Synthetic Chemical Biology Core Laboratory, Department of Medicinal Chemistry, University of Kansas, 2034 Becker Dr, Lawrence, KS 66047, USA

^c Department of BioEngineering University of Kansas, 1530 West 15th St., Lawrence, KS 66045, USA

^d Department of Mechanical Engineering, 3138 Learned Hall, 1530 West 15th St., Lawrence, KS 66045, USA

^e Department of Cancer Biology and KU Cancer Center, University of Kansas Medical Center, Kansas City, KS, USA

Abstract

We detail a heterobifunctional, 7-aminocoumarin photocleavable (PC) linker with unique properties to covalently attach Abs to surfaces and subsequently release them with visible light (400–450 nm). The PC linker allowed rapid (2 min) and efficient (>90%) release of CTCs and EVs without damaging their molecular cargo.

Chemical Communications

Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment†

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Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment

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