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Issue 16, 2020
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Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

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Abstract

A highly sensitive laser-induced fluorescence detection system with real-time imaging focusing instead of the use of fluorescent reagents were developed for the detection of analytes in nanocapillaries. A cylindrical lens was adopted for the shaping of a laser beam to increase its spatial resolution. The limit of detection with a capillary having a radius of 440 nm was 6.76 yoctomoles (or four molecules) for fluorescein, and 84.5 yoctomoles (or 51 molecules) for FAM-labeled Hsa-miR-17. The designed system exhibited high sensitivity and stability for femtoliters of sample and was hence indicated to be suitable for analyses of single cells.

Graphical abstract: Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

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Supplementary files

Article information


Submitted
11 Dec 2019
Accepted
14 Jan 2020
First published
14 Jan 2020

Chem. Commun., 2020,56, 2423-2426
Article type
Communication

Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

W. Zhang, L. Liu, Q. Zhang, D. Zhang, Q. Hu, Y. Wang, X. Wang, Q. Pu and G. Guo, Chem. Commun., 2020, 56, 2423
DOI: 10.1039/C9CC09594B

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