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Issue 17, 2020
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Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

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Abstract

Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex–cation interactions in RNA.

Graphical abstract: Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

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Article information


Submitted
03 Dec 2019
Accepted
29 Jan 2020
First published
29 Jan 2020

Chem. Commun., 2020,56, 2634-2637
Article type
Communication

Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

J. C. Savage, M. A. Davare and U. Shinde, Chem. Commun., 2020, 56, 2634
DOI: 10.1039/C9CC09375C

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