Issue 21, 2020
From the journal:

Chemical Communications

The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols†

Jeremy David Adams, ORCID logo a   Joachim Justad Røise, ORCID logo b   David Sam Lee ORCID logo c  and  Niren Murthy ORCID logo *de  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA

b Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA

c Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, USA

d Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
E-mail: nmurthy@berkeley.edu

e Innovative Genomics Institute (IGI), 2151 Berkeley Way, Berkeley, CA 94704, USA

Abstract

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.

Graphical abstract: The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9CC09136J
Article type
Communication
Submitted
23 Nov 2019
Accepted
10 Feb 2020
First published
10 Feb 2020

Chem. Commun., 2020,56, 3175-3178

Permissions

Request permissions

The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols

J. D. Adams, J. J. Røise, D. S. Lee and N. Murthy, Chem. Commun., 2020, 56, 3175 DOI: 10.1039/C9CC09136J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements