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The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols

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Abstract

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.

Graphical abstract: The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols

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Article information


Submitted
23 Nov 2019
Accepted
10 Feb 2020
First published
10 Feb 2020

Chem. Commun., 2020, Advance Article
Article type
Communication

The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols

J. D. Adams, J. J. Røise, D. S. Lee and N. Murthy, Chem. Commun., 2020, Advance Article , DOI: 10.1039/C9CC09136J

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