Jump to main content
Jump to site search

Issue 10, 2020
Previous Article Next Article

Complete identification of all 20 relevant epimeric peptides in β-amyloid: a new HPLC-MS based analytical strategy for Alzheimer's research

Author affiliations

Abstract

Although the underlying cause of Alzheimer's disease (AD) is not known, the extracellular deposition of β-amyloid (Aβ) is considered as a hallmark of AD brains. Evidence has shown the occurrence of D-Asp, isoAsp, and D-Ser residues in Aβ, which may be indicative of and/or contribute to the neurodegeneration in AD patients. Herein, we have developed the first high-throughput profiling technique for all 20 isobaric Aβ peptide epimers containing Asp, isoAsp, and Ser isomers using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This new analytical strategy allows the direct detection and identification of all possible Asp, isoAsp, and Ser stereoisomers in Aβ, and may contribute to a better understanding of the pathogenesis of AD.

Graphical abstract: Complete identification of all 20 relevant epimeric peptides in β-amyloid: a new HPLC-MS based analytical strategy for Alzheimer's research

Back to tab navigation

Supplementary files

Article information


Submitted
21 Nov 2019
Accepted
03 Jan 2020
First published
10 Jan 2020

Chem. Commun., 2020,56, 1537-1540
Article type
Communication

Complete identification of all 20 relevant epimeric peptides in β-amyloid: a new HPLC-MS based analytical strategy for Alzheimer's research

S. Du, E. R. Readel, M. Wey and D. W. Armstrong, Chem. Commun., 2020, 56, 1537
DOI: 10.1039/C9CC09080K

Social activity

Search articles by author

Spotlight

Advertisements