Fluorescence nanosensor for in situ detection of phosphate and alkaline phosphatase in parathyroid dysfunction mice
Parathyroid dysfunction can lead to serious complications that mainly divided into two types including hyperparathyroidism and hypoparathyroidism. Patients with hyperparathyroidism have increased alkaline phosphatase (ALP) levels, while patients with hypoparathyroidism have decreased ALP levels. Notably, abnormal levels of phosphate are associated with the parathyroid dysfunction. Therefore, the type of parathyroid dysfunction can be initially determined by detecting phosphate and ALP levels. Herein, we designed and prepared a MOF-based nanosensor to achieve simultaneous detection of phosphate and ALP. The Zr (IV) as the active center of nano-MOF can specifically bind the phosphate, thus achieved selectively phosphate’s fluorescence sensing. The p-nitrophenyl phosphate (PNPP), as the ALP substrate molecule, was stably attached to the surface of MOFs by self-assembly, achieving ALP-specific recognition. We established mice models of hyperparathyroidism and hypoparathyroidism. The mice in vivo imaging study of ALP and blood phosphorus levels enable to distinguish of the two types of disease model mice from normal mice. The present work provides a powerful tool for the early diagnosis of parathyroid disease.