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Issue 14, 2020
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Nucleoside-modified AdoMet analogues for differential methyltransferase targeting

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Abstract

Methyltransferases (MTases) modify a wide range of biomolecules using S-adenosyl-L-methionine (AdoMet) as the cosubstrate. Synthetic AdoMet analogues are powerful tools to site-specifically introduce a variety of functional groups and exhibit potential to be converted only by distinct MTases. Extending the size of the substituent at the sulfur/selenium atom provides selectivity among MTases but is insufficient to discriminate between promiscuous MTases. We present a panel of AdoMet analogues differing in the nucleoside moiety (NM-AdoMets). These NM-AdoMets were efficiently produced by a previously uncharacterized methionine adenosyltransferase (MAT) from methionine and ATP analogues, such as ITP and N6-propargyl-ATP. The N6-modification changed the relative activity of three representative MTases up to 13-fold resulting in discrimination of substrates for the methyl transfer and could also be combined with transfer of allyl and propargyl groups.

Graphical abstract: Nucleoside-modified AdoMet analogues for differential methyltransferase targeting

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Supplementary files

Article information


Submitted
08 Oct 2019
Accepted
19 Nov 2019
First published
22 Jan 2020

This article is Open Access

Chem. Commun., 2020,56, 2115-2118
Article type
Communication

Nucleoside-modified AdoMet analogues for differential methyltransferase targeting

N. V. Cornelissen, F. Michailidou, F. Muttach, K. Rau and A. Rentmeister, Chem. Commun., 2020, 56, 2115
DOI: 10.1039/C9CC07807J

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