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Illuminating glycoscience: synthetic strategies for FRET-enabled carbohydrate active enzyme probes

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Abstract

Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyse their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their underpinning physiological roles. As such, there is a considerable current requirement to be able to monitor, quantify and inhibit CAZy activity. It is here that Förster resonance energy transfer (FRET) is emerging as a powerful tool in enabling this, through synthetic conjugation of appropriate fluorogenic partners into a particular CAZy substrate. In this review we survey recent accomplishments in synthetic methodology for accessing defined carbohydrate structures, suitably equipped with FRET probe capability, followed by their utilisation in studying particular classes of CAZy. The majority of examples concern endo-acting glycosidases, but emergent probes for exo-glycosidases, glycosyl transferases and catalytic antibodies are also examined.

Graphical abstract: Illuminating glycoscience: synthetic strategies for FRET-enabled carbohydrate active enzyme probes

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Article information


Submitted
24 Jul 2020
Accepted
02 Sep 2020
First published
17 Sep 2020

This article is Open Access

RSC Chem. Biol., 2020, Advance Article
Article type
Review Article

Illuminating glycoscience: synthetic strategies for FRET-enabled carbohydrate active enzyme probes

M. Singh, M. Watkinson, E. M. Scanlan and G. J. Miller, RSC Chem. Biol., 2020, Advance Article , DOI: 10.1039/D0CB00134A

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