Self-assembled cell sheets composed of mesenchymal stem cell and gelatin nanofiber for treatment of full thickness wounds
Cell sheets engineering received great attention because of the thin layers of tissue can be easily transplanted to defect sites. Wound dressing materials are required to support fast re-epithelization both with keratinocytes and fibroblasts to enhanced prognosis and therapeutic outcomes. We prepared self-assembled cell sheets composed of adipocyte derived stem cells (ADSCs) and surface-engineered nanofibrils (NF). NF was surface-engineered with multilayers of gelatin so that cell sheets could spontaneously assembled within 3 days in cell culture plates. Dorsal wounds transplanted with the cell sheets exhibited higher wound healing rates when high concentration of gelatin was immobilized on the surfaces of NF. Histochemical staining revealed that those with gelatin-immobilized NF showed higher expression of cytokeratin and collagen in re-epithelized epidermis. Keratinocytic differentiation of epidermis was molecularly evidenced by higher expression of keratinocyte-specific genes.