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Self-assembled cell sheets composed of mesenchymal stem cell and gelatin nanofiber for treatment of full thickness wounds

Abstract

Cell sheets engineering received great attention because of the thin layers of tissue can be easily transplanted to defect sites. Wound dressing materials are required to support fast re-epithelization both with keratinocytes and fibroblasts to enhanced prognosis and therapeutic outcomes. We prepared self-assembled cell sheets composed of adipocyte derived stem cells (ADSCs) and surface-engineered nanofibrils (NF). NF was surface-engineered with multilayers of gelatin so that cell sheets could spontaneously assembled within 3 days in cell culture plates. Dorsal wounds transplanted with the cell sheets exhibited higher wound healing rates when high concentration of gelatin was immobilized on the surfaces of NF. Histochemical staining revealed that those with gelatin-immobilized NF showed higher expression of cytokeratin and collagen in re-epithelized epidermis. Keratinocytic differentiation of epidermis was molecularly evidenced by higher expression of keratinocyte-specific genes.

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Supplementary files

Article information


Submitted
02 Jun 2020
Accepted
23 Jun 2020
First published
27 Jun 2020

Biomater. Sci., 2020, Accepted Manuscript
Article type
Paper

Self-assembled cell sheets composed of mesenchymal stem cell and gelatin nanofiber for treatment of full thickness wounds

O. Pham-Nguyen, J. U. Shin, H. Kim and H. S. Yoo, Biomater. Sci., 2020, Accepted Manuscript , DOI: 10.1039/D0BM00910E

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