Jump to main content
Jump to site search

Issue 18, 2020
Previous Article Next Article

NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice

Author affiliations

Abstract

Second near infrared (NIR-II) window fluorescence imaging between 1000 and 1700 nm with reduced scattering and autofluorescence and deep tissue light penetration allows early and non-invasive determination of vascular pathologies. Here, we demonstrate in vivo NIR-II imaging techniques for tracking hyperglycaemia-induced Intracerebral Hemorrhage (ICH) and Blood Brain Barrier (BBB) hyperpermeability in Cerebral Cavernous Malformation (CCM) deficient mice (CCM1+/−). We synthesised PEGylated Ag2S quantum dots (QDs) with a bright fluorescent emission peak centred at 1135 nm under an 808 nm NIR light for dynamic imaging of cerebral vasculature in mice and determined the development of ICH and BBB impairment in hyperglycaemic CCM1+/− mice. In vivo optical imaging was conducted with micro-CT (including k-mean cluster analysis) as well as in vivo permeability assays using FITC-dextran perfusion and IgG staining, respectively. The increased BBB permeability in CCM1+/− mice was further demonstrated to be associated with a high-glucose-caused decrease of CCM1 expressions. This study validates that deep-penetrating NIR-II QDs can be used for the tracking of ICH and BBB hyperpermeability in transgenic mice models of cerebral vascular anomalies.

Graphical abstract: NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice

Back to tab navigation

Supplementary files

Article information


Submitted
26 May 2020
Accepted
04 Aug 2020
First published
05 Aug 2020

Biomater. Sci., 2020,8, 5133-5144
Article type
Paper

NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice

A. K. Parchur, Z. Fang, J. M. Jagtap, G. Sharma, C. Hansen, S. Shafiee, W. Hu, Q. R. Miao and A. Joshi, Biomater. Sci., 2020, 8, 5133
DOI: 10.1039/D0BM00873G

Social activity

Search articles by author

Spotlight

Advertisements