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Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

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Abstract

The aim of this study was to investigate the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on novel thermosensitive in situ forming hydrogel systems comprising methoxy polyethylene glycol-polycaprolactone (MP) and RGD-conjugated MP (MP–RGD) in vitro and in vivo. Real-time polymerase chain reaction (PCR) together with immunofluorescence staining revealed the strong expression of osteogenic markers (collagen 1 and osteocalcin) of BMSCs in MP/MP–RGD samples compared to MP samples. PCR array testing also showed the upregulation of the interconnected signaling networks regulating cell proliferation and differentiation, which was further verified through the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Histological findings and computed tomographic analysis demonstrated that the MP/MP–RGD hydrogel dramatically promoted new bone formation in a rabbit calvarial defect model. In conclusion, this hydrogel appears to elicit cellular behaviors desired for bone tissue regeneration.

Graphical abstract: Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

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Supplementary files

Article information


Submitted
14 Apr 2020
Accepted
28 May 2020
First published
08 Jun 2020

Biomater. Sci., 2020, Advance Article
Article type
Paper

Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

H. J. Kim, S. J. You, D. H. Yang, J. Eun, H. K. Park, M. S. Kim and H. J. Chun, Biomater. Sci., 2020, Advance Article , DOI: 10.1039/D0BM00588F

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