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Customized hydrogel substrates for serum-free expansion of functional hMSCs

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Abstract

We describe a screening approach to identify customized substrates for serum-free human mesenchymal stromal cell (hMSC) culture. In particular, we combine a biomaterials screening approach with design of experiments (DOE) and multivariate analysis (MVA) to understand the effects of substrate stiffness, substrate adhesivity, and media composition on hMSC behavior in vitro. This approach enabled identification of poly(ethylene glycol)-based and integrin binding hydrogel substrate compositions that supported functional hMSC expansion in multiple serum-containing and serum-free media, as well as the expansion of MSCs from multiple, distinct sources. The identified substrates were compatible with standard thaw, seed, and harvest protocols. Finally, we used MVA on the screening data to reveal the importance of serum and substrate stiffness on hMSC expansion, highlighting the need for customized cell culture substrates in optimal hMSC biomanufacturing processes.

Graphical abstract: Customized hydrogel substrates for serum-free expansion of functional hMSCs

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Supplementary files

Article information


Submitted
03 Apr 2020
Accepted
07 Jun 2020
First published
10 Jun 2020

Biomater. Sci., 2020, Advance Article
Article type
Paper

Customized hydrogel substrates for serum-free expansion of functional hMSCs

N. N. T. Le, T. L. Liu, J. Johnston, J. D. Krutty, K. M. Templeton, V. Harms, A. Dias, H. Le, P. Gopalan and W. L. Murphy, Biomater. Sci., 2020, Advance Article , DOI: 10.1039/D0BM00540A

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