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Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

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Abstract

Gram-negative bacteria are a significant cause of infections acquired in both hospital and community settings, resulting in a high mortality rate worldwide. Currently, a Gram-negative infection is diagnosed by symptom evaluation and is treated with empiric antibiotics which target both Gram-negative and Gram-positive bacteria. A rapid and simple diagnostic method would enable immediate and targeted treatment, while dramatically reducing antibiotic overuse. Herein, we introduce a method utilizing a fluorescent derivative of colistin (COL-FL), that can directly label the Gram-negative cell wall of live bacteria and universally detect the targets within 10 min. By using the COL-FL assay, we achieved the differential labeling of various Gram-negative pathogens related to hospital-acquired infections, which could be subsequently detected via spectrofluorometry and microscopy. Further, we determined that our method can be used for complex samples, such as combinations of multiple bacterial types; bacteria in the presence of mammalian cells; and bacteria with serum components. This assay can be integrated into a simple diagnostic platform for rapid screening tests and the stratification of Gram-negative bacterial infections in the clinic.

Graphical abstract: Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

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Article information


Submitted
29 Nov 2019
Accepted
11 Jan 2020
First published
14 Jan 2020

Biomater. Sci., 2020, Advance Article
Article type
Paper

Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

J. S. Ryu, S. H. Im, Y. K. Kang, Y. S. Kim and H. J. Chung, Biomater. Sci., 2020, Advance Article , DOI: 10.1039/C9BM01926J

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