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Genetic recombination of poly(l-lysine) functionalized apoferritin nanocages that resemble viral capsid nanometer-sized platforms for gene therapy

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Abstract

Currently, bioengineered apoferritin nanocages with flexible protein shells and functionalized modifications have become an attractive approach for efficient anti-tumor therapy. Here, we modified the N-terminus of H-chain subunits in apoferritin with different amounts of lysine via genetic recombination to obtain a poly(L-lysine) modified H-chain apoferritin (nL-HFn) nanocage for siRNA delivery and gene therapy. To achieve excellent cellular affinity and uptake, the nanocarriers were internalized through transferrin receptor-mediated endocytosis, then escaped from the endosome for cytoplasmic transport. Compared with natural apoferritin, the siRNA-loaded genetic recombination NPs modified with lysine exhibit stronger RNA-interference and antitumor efficiency both in vitro and in 4T1 tumor model mice. Therefore, bioengineered apoferritin nanocages modified with lysine might be a promising platform for nucleic acid drug delivery.

Graphical abstract: Genetic recombination of poly(l-lysine) functionalized apoferritin nanocages that resemble viral capsid nanometer-sized platforms for gene therapy

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Article information


Submitted
12 Nov 2019
Accepted
17 Jan 2020
First published
03 Feb 2020

Biomater. Sci., 2020, Advance Article
Article type
Paper

Genetic recombination of poly(L-lysine) functionalized apoferritin nanocages that resemble viral capsid nanometer-sized platforms for gene therapy

H. Huang, S. Yuan, Z. Ma, P. Ji, X. Ma, Z. Wu and X. Qi, Biomater. Sci., 2020, Advance Article , DOI: 10.1039/C9BM01822K

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