Issue 47, 2020

A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions

Abstract

Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. A ratiometric film sensor based on gold nanoclusters (AuNCs) was fabricated for highly sensitive determination of free bilirubin (fBR). Using bovine serum albumin (BSA) as a template, AuNCs that can emit blue and red fluorescence were prepared by the hydrothermal method at different pH values. Two kinds of AuNCs were incorporated into a single film by the layer-by-layer assembly (LBL) technique. The obtained thin-film showed dual fluorescence peaks excited at 372 nm, corresponding to the blue (443 nm) and red (622 nm) emissions of AuNCs respectively. When fBR interacted with the film, both fluorescence peaks were quenched at different degrees. A ratiometric method for fBR detection was established based on the fluorescence intensity ratio of the two emissions. The linear calibration curve for fBR lay in the concentration range of 0.01–2.00 μmol L−1 with a detection limit of 8.90 ± 0.34 nmol L−1 (S/N = 3). The film sensor showed a quick and sensitive response to fBR and could detect fBR in real samples with satisfactory results.

Graphical abstract: A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions

Article information

Article type
Paper
Submitted
19 Sep 2020
Accepted
25 Oct 2020
First published
27 Oct 2020

Anal. Methods, 2020,12, 5691-5698

A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions

W. Xiao, D. Zhi, Q. Pan, Y. Liang, F. Zhou and Z. Chen, Anal. Methods, 2020, 12, 5691 DOI: 10.1039/D0AY01781G

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