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Rapid and highly sensitive detection of Salmonella Typhimurium in lettuce by using magnetic fluorescent nanoparticles

Abstract

The high-efficiency detection of Salmonella Typhimurium (S. Typhimurium), a common foodborne bacterial, is important for leafy vegetables safety assurance. In this study, a fluorescent sensor (FMNCs-Apt), based on Fe3O4 magnetic nanoparticles and aptamer-modified carbon quantum dots, was developed for rapid and highly sensitive detection of S. Typhimurium in lettuce. First, carbon quantum dots were covalently bonded to the surface of prepared Fe3O4@chitosan to form magnetic fluorescence composite nanoparticles (FMNCs). Then, the aptamers of S. Typhimurium were covalently linked to the surface FMNCs-Apt. Fluorescence intensity of the FMNCs-Apt probes decreased as they aggregated on the surface of bacterial and the aggregation was separated using a magnet. Under the optimal conditions, the fluorescence change values of the solution were shown a good linear relationship with the concentration of Salmonella (103 - 106 cfu·mL-1). The detection limit of the method is 100 cfu·mL-1 and 138 cfu·mL-1 in fresh-cut vegetables washing solution and lettuce sample respectively. Accordingly, this developed fluorescent probe became a highly sensitive and efficient sensor for rapid detection of S. Typhimurium in lettuce.

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Supplementary files

Article information


Submitted
13 Sep 2020
Accepted
06 Oct 2020
First published
17 Oct 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Paper

Rapid and highly sensitive detection of Salmonella Typhimurium in lettuce by using magnetic fluorescent nanoparticles

Z. Guo, X. Huang, Z. Li, J. Shi, X. Zhai, X. Hu, N. Liang and X. Zou, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/D0AY01744B

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